su 5402 Search Results


su5402  (MedChemExpress)
94
MedChemExpress su5402
( A ) Immunofluorescence staining for phosphorylated Smad1/5/9 in embryos injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 6 hpf. The injection site was determined by red fluorescence. Signal quantifications along the ventro-dorsal axis are displayed beside the merged images. ( B ) Immunofluorescence staining for pSmad1/5/9 in explants injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 10 hpf. Signal quantifications along the anterior–posterior axis are shown next to the merged images. Clones of bmp4 injected blastomere would stay at the injected end when they differentiated into enveloping layer cells. But when they differentiate into deep cells, they would involute and migrate anteriorly. Signal intensities of pSmad1/5/9 and RFP were normalized first by the DAPI signal and then by their mean signal intensity. ( C ) Whole mount in situ hybridization (WISH) of id1 (left) HCR co-staining of id1 , tbxta and tbx6 (middle) in Bmp4 explants at 14-18 hpf, with injection sites oriented to the right. Signal quantifications along the white dashed line are presented to the right. ( D ) WISH of eve1, fgf8a, sox32, cdx4, chrd in Bmp4 explants at 6 hpf, with injection sites oriented to the right. ( E ) WISH of tbxta, cdx4 in Bmp4 explants treated with Nodal inhibitor (SB505124, 50 μM), Wnt inhibitor (IWP-L6,25 μM) or FGF inhibitor <t>(SU5402,</t> 25 μM) at 6 hpf, with injection sites oriented to the right. Representative images of treated or untreated Bmp4 explants at 24 hpf are displayed. ( F ) WISH of wnt8a, ndr2 , and tbxta in uninjected, 20 pg bmp2b mRNA injected, 0.5 pg bmp4 mRNA injected explants or embryos at 6 hpf. ( G ) Expression patterns of fgf8a , sox17 , and eve1 revealed by WISH in bmp4 -injected embryos at 6 hpf. Views from the animal pole of embryos are displayed, with descendants of the bmp4 -injected blastomere labeled by DAB staining of GFP (left and middle images). ( H ) WISH of sox32 in uninjected, 8 pg or 20 pg bmp4 mRNA injected explants at 10 hpf. ( I ) Expression pattern of tbxta revealed by WISH in bmp4 -injected embryos at 12 hpf. ( J ) WISH for tbxta, egr2b, sox19a, olig2, foxd3, shha, tnnt2c, myod1, cldn3d, hoxc13b in bmp4 -injected embryos 24 hpf. ( K ) WISH for egr2b, gata6, shha, her1, fn1b , and foxd3 in Bmp4 explants at 24 hpf. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 µm.
Su5402, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/su+5402/SU+5402/pmc12759085-48-0-2
Average 94 stars, based on 1 article reviews
su5402 - by Bioz Stars, 2026-09
94/100 stars
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su5402  (Tocris)
95
Tocris su5402
( A ) Immunofluorescence staining for phosphorylated Smad1/5/9 in embryos injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 6 hpf. The injection site was determined by red fluorescence. Signal quantifications along the ventro-dorsal axis are displayed beside the merged images. ( B ) Immunofluorescence staining for pSmad1/5/9 in explants injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 10 hpf. Signal quantifications along the anterior–posterior axis are shown next to the merged images. Clones of bmp4 injected blastomere would stay at the injected end when they differentiated into enveloping layer cells. But when they differentiate into deep cells, they would involute and migrate anteriorly. Signal intensities of pSmad1/5/9 and RFP were normalized first by the DAPI signal and then by their mean signal intensity. ( C ) Whole mount in situ hybridization (WISH) of id1 (left) HCR co-staining of id1 , tbxta and tbx6 (middle) in Bmp4 explants at 14-18 hpf, with injection sites oriented to the right. Signal quantifications along the white dashed line are presented to the right. ( D ) WISH of eve1, fgf8a, sox32, cdx4, chrd in Bmp4 explants at 6 hpf, with injection sites oriented to the right. ( E ) WISH of tbxta, cdx4 in Bmp4 explants treated with Nodal inhibitor (SB505124, 50 μM), Wnt inhibitor (IWP-L6,25 μM) or FGF inhibitor <t>(SU5402,</t> 25 μM) at 6 hpf, with injection sites oriented to the right. Representative images of treated or untreated Bmp4 explants at 24 hpf are displayed. ( F ) WISH of wnt8a, ndr2 , and tbxta in uninjected, 20 pg bmp2b mRNA injected, 0.5 pg bmp4 mRNA injected explants or embryos at 6 hpf. ( G ) Expression patterns of fgf8a , sox17 , and eve1 revealed by WISH in bmp4 -injected embryos at 6 hpf. Views from the animal pole of embryos are displayed, with descendants of the bmp4 -injected blastomere labeled by DAB staining of GFP (left and middle images). ( H ) WISH of sox32 in uninjected, 8 pg or 20 pg bmp4 mRNA injected explants at 10 hpf. ( I ) Expression pattern of tbxta revealed by WISH in bmp4 -injected embryos at 12 hpf. ( J ) WISH for tbxta, egr2b, sox19a, olig2, foxd3, shha, tnnt2c, myod1, cldn3d, hoxc13b in bmp4 -injected embryos 24 hpf. ( K ) WISH for egr2b, gata6, shha, her1, fn1b , and foxd3 in Bmp4 explants at 24 hpf. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 µm.
Su5402, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/su+5402/SU+5402/pmc04169698-164-13-17
Average 95 stars, based on 1 article reviews
su5402 - by Bioz Stars, 2026-09
95/100 stars
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chemical treatment  (Tocris)
95
Tocris chemical treatment
( A ) Immunofluorescence staining for phosphorylated Smad1/5/9 in embryos injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 6 hpf. The injection site was determined by red fluorescence. Signal quantifications along the ventro-dorsal axis are displayed beside the merged images. ( B ) Immunofluorescence staining for pSmad1/5/9 in explants injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 10 hpf. Signal quantifications along the anterior–posterior axis are shown next to the merged images. Clones of bmp4 injected blastomere would stay at the injected end when they differentiated into enveloping layer cells. But when they differentiate into deep cells, they would involute and migrate anteriorly. Signal intensities of pSmad1/5/9 and RFP were normalized first by the DAPI signal and then by their mean signal intensity. ( C ) Whole mount in situ hybridization (WISH) of id1 (left) HCR co-staining of id1 , tbxta and tbx6 (middle) in Bmp4 explants at 14-18 hpf, with injection sites oriented to the right. Signal quantifications along the white dashed line are presented to the right. ( D ) WISH of eve1, fgf8a, sox32, cdx4, chrd in Bmp4 explants at 6 hpf, with injection sites oriented to the right. ( E ) WISH of tbxta, cdx4 in Bmp4 explants treated with Nodal inhibitor (SB505124, 50 μM), Wnt inhibitor (IWP-L6,25 μM) or FGF inhibitor <t>(SU5402,</t> 25 μM) at 6 hpf, with injection sites oriented to the right. Representative images of treated or untreated Bmp4 explants at 24 hpf are displayed. ( F ) WISH of wnt8a, ndr2 , and tbxta in uninjected, 20 pg bmp2b mRNA injected, 0.5 pg bmp4 mRNA injected explants or embryos at 6 hpf. ( G ) Expression patterns of fgf8a , sox17 , and eve1 revealed by WISH in bmp4 -injected embryos at 6 hpf. Views from the animal pole of embryos are displayed, with descendants of the bmp4 -injected blastomere labeled by DAB staining of GFP (left and middle images). ( H ) WISH of sox32 in uninjected, 8 pg or 20 pg bmp4 mRNA injected explants at 10 hpf. ( I ) Expression pattern of tbxta revealed by WISH in bmp4 -injected embryos at 12 hpf. ( J ) WISH for tbxta, egr2b, sox19a, olig2, foxd3, shha, tnnt2c, myod1, cldn3d, hoxc13b in bmp4 -injected embryos 24 hpf. ( K ) WISH for egr2b, gata6, shha, her1, fn1b , and foxd3 in Bmp4 explants at 24 hpf. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 µm.
Chemical Treatment, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/su+5402/SU+5402/pm29291977-250-19-24
Average 95 stars, based on 1 article reviews
chemical treatment - by Bioz Stars, 2026-09
95/100 stars
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fgfr inhibitor su5402  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology fgfr inhibitor su5402
( A ) Immunofluorescence staining for phosphorylated Smad1/5/9 in embryos injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 6 hpf. The injection site was determined by red fluorescence. Signal quantifications along the ventro-dorsal axis are displayed beside the merged images. ( B ) Immunofluorescence staining for pSmad1/5/9 in explants injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 10 hpf. Signal quantifications along the anterior–posterior axis are shown next to the merged images. Clones of bmp4 injected blastomere would stay at the injected end when they differentiated into enveloping layer cells. But when they differentiate into deep cells, they would involute and migrate anteriorly. Signal intensities of pSmad1/5/9 and RFP were normalized first by the DAPI signal and then by their mean signal intensity. ( C ) Whole mount in situ hybridization (WISH) of id1 (left) HCR co-staining of id1 , tbxta and tbx6 (middle) in Bmp4 explants at 14-18 hpf, with injection sites oriented to the right. Signal quantifications along the white dashed line are presented to the right. ( D ) WISH of eve1, fgf8a, sox32, cdx4, chrd in Bmp4 explants at 6 hpf, with injection sites oriented to the right. ( E ) WISH of tbxta, cdx4 in Bmp4 explants treated with Nodal inhibitor (SB505124, 50 μM), Wnt inhibitor (IWP-L6,25 μM) or FGF inhibitor <t>(SU5402,</t> 25 μM) at 6 hpf, with injection sites oriented to the right. Representative images of treated or untreated Bmp4 explants at 24 hpf are displayed. ( F ) WISH of wnt8a, ndr2 , and tbxta in uninjected, 20 pg bmp2b mRNA injected, 0.5 pg bmp4 mRNA injected explants or embryos at 6 hpf. ( G ) Expression patterns of fgf8a , sox17 , and eve1 revealed by WISH in bmp4 -injected embryos at 6 hpf. Views from the animal pole of embryos are displayed, with descendants of the bmp4 -injected blastomere labeled by DAB staining of GFP (left and middle images). ( H ) WISH of sox32 in uninjected, 8 pg or 20 pg bmp4 mRNA injected explants at 10 hpf. ( I ) Expression pattern of tbxta revealed by WISH in bmp4 -injected embryos at 12 hpf. ( J ) WISH for tbxta, egr2b, sox19a, olig2, foxd3, shha, tnnt2c, myod1, cldn3d, hoxc13b in bmp4 -injected embryos 24 hpf. ( K ) WISH for egr2b, gata6, shha, her1, fn1b , and foxd3 in Bmp4 explants at 24 hpf. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 µm.
Fgfr Inhibitor Su5402, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/su+5402/SU+5402/pmc03809791-399-1-8
Average 93 stars, based on 1 article reviews
fgfr inhibitor su5402 - by Bioz Stars, 2026-09
93/100 stars
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su 5402  (Cayman Chemical)
N/A
SU 5402 is an inhibitor of the tyrosine kinase domains of VEGFR2 FGFR1 and PDGFRβ ICs 0 02 0 03 and 0 51 µM respectively It is much less effective against other receptor tyrosine kinases
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su 5402 2-hydroxyethyl ester  (Biosynth Carbosynth)
N/A
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5-ethoxycarbonyl su 5402 methyl ester  (Toronto Research Chemicals)
N/A
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su 5402  (Toronto Research Chemicals)
N/A
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5-carboxy su 5402  (Toronto Research Chemicals)
N/A
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su-5402 2-hydroxyethyl ester  (Santa Cruz Biotechnology)
N/A
SU-5402 2-Hydroxyethyl Ester is an SU 5402 production intermediate
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su-5402  (Aladdin Scientific Corporation)
N/A
Product Describtion:SU 5402 is a potent multi-targeted receptor tyrosine kinase inhibitor with IC50 of 20 nM, 30 nM, and 510 nM for VEGFR2, FGFR1, and PDGFRβ, respectively.
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Image Search Results


( A ) Immunofluorescence staining for phosphorylated Smad1/5/9 in embryos injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 6 hpf. The injection site was determined by red fluorescence. Signal quantifications along the ventro-dorsal axis are displayed beside the merged images. ( B ) Immunofluorescence staining for pSmad1/5/9 in explants injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 10 hpf. Signal quantifications along the anterior–posterior axis are shown next to the merged images. Clones of bmp4 injected blastomere would stay at the injected end when they differentiated into enveloping layer cells. But when they differentiate into deep cells, they would involute and migrate anteriorly. Signal intensities of pSmad1/5/9 and RFP were normalized first by the DAPI signal and then by their mean signal intensity. ( C ) Whole mount in situ hybridization (WISH) of id1 (left) HCR co-staining of id1 , tbxta and tbx6 (middle) in Bmp4 explants at 14-18 hpf, with injection sites oriented to the right. Signal quantifications along the white dashed line are presented to the right. ( D ) WISH of eve1, fgf8a, sox32, cdx4, chrd in Bmp4 explants at 6 hpf, with injection sites oriented to the right. ( E ) WISH of tbxta, cdx4 in Bmp4 explants treated with Nodal inhibitor (SB505124, 50 μM), Wnt inhibitor (IWP-L6,25 μM) or FGF inhibitor (SU5402, 25 μM) at 6 hpf, with injection sites oriented to the right. Representative images of treated or untreated Bmp4 explants at 24 hpf are displayed. ( F ) WISH of wnt8a, ndr2 , and tbxta in uninjected, 20 pg bmp2b mRNA injected, 0.5 pg bmp4 mRNA injected explants or embryos at 6 hpf. ( G ) Expression patterns of fgf8a , sox17 , and eve1 revealed by WISH in bmp4 -injected embryos at 6 hpf. Views from the animal pole of embryos are displayed, with descendants of the bmp4 -injected blastomere labeled by DAB staining of GFP (left and middle images). ( H ) WISH of sox32 in uninjected, 8 pg or 20 pg bmp4 mRNA injected explants at 10 hpf. ( I ) Expression pattern of tbxta revealed by WISH in bmp4 -injected embryos at 12 hpf. ( J ) WISH for tbxta, egr2b, sox19a, olig2, foxd3, shha, tnnt2c, myod1, cldn3d, hoxc13b in bmp4 -injected embryos 24 hpf. ( K ) WISH for egr2b, gata6, shha, her1, fn1b , and foxd3 in Bmp4 explants at 24 hpf. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 µm.

Journal: The EMBO Journal

Article Title: BMP4 initiates and patterns ventral-caudal structures in zebrafish and human pluripotent stem cell aggregates

doi: 10.1038/s44318-025-00643-6

Figure Lengend Snippet: ( A ) Immunofluorescence staining for phosphorylated Smad1/5/9 in embryos injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 6 hpf. The injection site was determined by red fluorescence. Signal quantifications along the ventro-dorsal axis are displayed beside the merged images. ( B ) Immunofluorescence staining for pSmad1/5/9 in explants injected with bmp4 + RFP mRNA (left) and RFP mRNA alone (right) at 10 hpf. Signal quantifications along the anterior–posterior axis are shown next to the merged images. Clones of bmp4 injected blastomere would stay at the injected end when they differentiated into enveloping layer cells. But when they differentiate into deep cells, they would involute and migrate anteriorly. Signal intensities of pSmad1/5/9 and RFP were normalized first by the DAPI signal and then by their mean signal intensity. ( C ) Whole mount in situ hybridization (WISH) of id1 (left) HCR co-staining of id1 , tbxta and tbx6 (middle) in Bmp4 explants at 14-18 hpf, with injection sites oriented to the right. Signal quantifications along the white dashed line are presented to the right. ( D ) WISH of eve1, fgf8a, sox32, cdx4, chrd in Bmp4 explants at 6 hpf, with injection sites oriented to the right. ( E ) WISH of tbxta, cdx4 in Bmp4 explants treated with Nodal inhibitor (SB505124, 50 μM), Wnt inhibitor (IWP-L6,25 μM) or FGF inhibitor (SU5402, 25 μM) at 6 hpf, with injection sites oriented to the right. Representative images of treated or untreated Bmp4 explants at 24 hpf are displayed. ( F ) WISH of wnt8a, ndr2 , and tbxta in uninjected, 20 pg bmp2b mRNA injected, 0.5 pg bmp4 mRNA injected explants or embryos at 6 hpf. ( G ) Expression patterns of fgf8a , sox17 , and eve1 revealed by WISH in bmp4 -injected embryos at 6 hpf. Views from the animal pole of embryos are displayed, with descendants of the bmp4 -injected blastomere labeled by DAB staining of GFP (left and middle images). ( H ) WISH of sox32 in uninjected, 8 pg or 20 pg bmp4 mRNA injected explants at 10 hpf. ( I ) Expression pattern of tbxta revealed by WISH in bmp4 -injected embryos at 12 hpf. ( J ) WISH for tbxta, egr2b, sox19a, olig2, foxd3, shha, tnnt2c, myod1, cldn3d, hoxc13b in bmp4 -injected embryos 24 hpf. ( K ) WISH for egr2b, gata6, shha, her1, fn1b , and foxd3 in Bmp4 explants at 24 hpf. Each experiment was performed for at least three independent replicates (technical replicates). Scale bars: 100 µm.

Article Snippet: SU5402 , MedChemExpress , HY-10407.

Techniques: Immunofluorescence, Staining, Injection, Fluorescence, Clone Assay, In Situ Hybridization, Expressing, Labeling